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IL-10 and TGF-beta production by NK cells in patients with multiple sclerosis.
We have examined the cytokine production of natural killer (NK) cells in multiple sclerosis (MS) patients and have evaluated their capacity to influence the proliferation of T cells. Cytokine production by unstimulated NK cells and the effects of NK cell-conditioned supernatants on proliferation of blood lymphocytes were studied in patients with MS (n = 32) and in normal controls (n = 25). In addition, production of both Th1 and Th2 cytokines was evaluated in NK cells from patients with MS (n = 15). Mononuclear cells from patients with MS were activated with PHA or with PMA and ionomycin, and NK cells were separated from PBL by counterflow centrifugal elutriation. Interleukin-2 (IL-2), IL-4, and interferon (IFN)-gamma were measured in the supernatants of whole cultures and in supernatants of NK cells after stimulation. By whole cultures, NK cells from MS patients produced less IL-10 (p < 0.01) and TGF

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