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The dual effects of cytoplasmic Ca(2+) signals on mitosis make this phase of the cell cycle a potential target of anti-mitotic agents. One family of calcium-stimulated proteins that plays an important role in the regulation of the cell cycle is the SR/ER Ca(2+)-release channel. In the present study, the anti-mitotic effects of several G2-selective compounds were tested in two cell lines: PC12 cells that express store-operated Ca(2+) channels (SOCs) and Rat-1 cells that do not express any form of Ca(2+) release channels. In these two cell lines, it was first demonstrated that G2-arrested cells fail to mount a Ca(2+) response in response to thapsigargin, an inhibitor of the store-operated Ca(2+) channel (SOC). The compounds tested were able to block the Ca(2+) response to thapsigargin in G2-arrested Rat-1 cells, but not in G2-arrested PC12 cells. These results show that the cell cycle is not the only parameter that can be used for screening anti-mitotic compounds.Isolation of cold-active beta-glucosidase from a cold-adapted bacterium
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